Application
Western blot
Sample
Human Cell lysate - whole cell (brain)
Loading amount
60 µg
Specification
brain
Treatment
Irradiation 0, 2, 4,6 Gy for 24 h
Gel Running Conditions
Reduced Denaturing (4-12 % gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % BSA + 0,1 % tween in TBS
Secondary antibody
Name
Non-Abcam antibody was used: cell signaling
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~ 15 kDa
Positive control
none
Additional data
Additional Notes
Too strong background also when antibody dilution was set to 1:10000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 18 2013