Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
A very low dilution factor compared with that for mouse and rat testis, under identical conditions.
However, apart from the Leydig cells, pattern of positivity is similar.
Also, ab2893 gives a remarkably similar pattern of staining.
Submitted image shows, using coloured arrowheads, three distinct patterns of positivity: light cytoplasmic in Leydig cells (green) dense nuclear ( black) eccentric nuclear dot (red).
As positivity is known to be nuclear, I assume that the Leydig cell positivity is non-specific. However, ab2893 also immunostains Leydig cell cytoplasm.
This testis is a younger sample than that used for abreview of ab2893 (senescent testis)
However, apart from the Leydig cells, pattern of positivity is similar.
Also, ab2893 gives a remarkably similar pattern of staining.
Submitted image shows, using coloured arrowheads, three distinct patterns of positivity: light cytoplasmic in Leydig cells (green) dense nuclear ( black) eccentric nuclear dot (red).
As positivity is known to be nuclear, I assume that the Leydig cell positivity is non-specific. However, ab2893 also immunostains Leydig cell cytoplasm.
This testis is a younger sample than that used for abreview of ab2893 (senescent testis)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 May 15 2013