概述

  • 产品名称Anti-GALNT7抗体
    参阅全部 GALNT7 一抗
  • 描述
    兔多克隆抗体to GALNT7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human GALNT7 aa 600 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q80VA0

  • 阳性对照
    • This antibody gave a positive signal in Mouse Colon Tissue Lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 75 kDa).

靶标

  • 功能Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.
  • 组织特异性Widely expressed. Expressed in uterus, retina, kidney, small intestine, omentum, stomach and CNS.
  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • 结构域There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GalNAc T7 antibody
    • GalNAc-T7 antibody
    • GalNAcT7 antibody
    • GALNT 7 antibody
    • Galnt7 antibody
    • GALT7_HUMAN antibody
    • N acetylgalactosaminyltransferase 7 antibody
    • N-acetylgalactosaminyltransferase 7 antibody
    • Polypeptide GalNAc transferase 7 antibody
    • Polypeptide N acetylgalactosaminyltransferase 7 antibody
    • pp GaNTase 7 antibody
    • pp-GaNTase 7 antibody
    • Protein UDP acetylgalactosaminyltransferase 7 antibody
    • Protein-UDP acetylgalactosaminyltransferase 7 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 7 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 7 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 antibody
    see all

Anti-GALNT7 antibody 图像

  • Anti-GALNT7 antibody (ab113743) at 1 µg/ml (Milk blocking (5%)) + Colon (Mouse) Tissue Lysate at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Additional bands at : 53 kDa (possible non-specific binding),76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab113743 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-GALNT7 antibody (ab113743)参考文献

ab113743 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113743.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"