Anti-GALNT3抗体(ab113880)

概述

  • 产品名称Anti-GALNT3抗体
    参阅全部 GALNT3 一抗
  • 描述
    兔多克隆抗体to GALNT3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 93-142 (PERPCLQGYY TAAELKPVLD PPQDSNAPGA GKPFKITHLS EEQKEKE) of Rat GALNT3 (NP_001015032).

  • 阳性对照
    • Rat brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Glycosylates FGF23. Plays a central role in phosphate homeostasis.
  • 组织特异性Expressed in organs that contain secretory epithelial glands. Highly expressed in pancreas, skin, kidney and testis. Weakly expressed in prostate, ovary, intestine and colon. Also expressed in placenta and lung and fetal lung and fetal kidney.
  • 通路Protein modification; protein glycosylation.
  • 疾病相关Defects in GALNT3 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • 序列相似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • 结构域There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • 细胞定位Golgi apparatus > Golgi stack membrane. Resides preferentially in the trans and medial parts of the Golgi stack.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686C10199 antibody
    • EC 2.4.1.41 antibody
    • GalNAc T3 antibody
    • GalNAc transferase 3 antibody
    • GalNAc-T3 antibody
    • GalNAcT3 antibody
    • GALNT3 antibody
    • GALT3_HUMAN antibody
    • HFTC antibody
    • HHS antibody
    • MGC61909 antibody
    • OTTHUMP00000204915 antibody
    • OTTHUMP00000204919 antibody
    • Polypeptide GalNAc transferase 3 antibody
    • Polypeptide GalNAc transferase T3 antibody
    • Polypeptide N acetylgalactosaminyltransferase 3 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 3 antibody
    • pp-GaNTase 3 antibody
    • ppGaNTase 3 antibody
    • Protein UDP acetylgalactosaminyltransferase 3 antibody
    • Protein-UDP acetylgalactosaminyltransferase 3 antibody
    • RP23-306G16.2 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 3 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 3 (GalNAc T3) antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 antibody
    • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 antibody
    see all

Anti-GALNT3 antibody 图像

  • Anti-GALNT3 antibody (ab113880) at 1 µg/ml + Rat brain lysate at 10 µg

    Predicted band size : 73 kDa

Anti-GALNT3 antibody (ab113880)参考文献

ab113880 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (lysate)
Specification lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

提交于 Oct 27 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (colon and breast cancer)
Loading amount 50 µg
Specification colon and breast cancer
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"