Anti-GALNT2抗体(ab102650)

概述

  • 产品名称Anti-GALNT2抗体
    参阅全部 GALNT2 一抗
  • 描述
    兔多克隆抗体to GALNT2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 32-62 (

    AGGAGGGAGRKEDWNEIDPIKKKDLHHSNGE

    ) of Human GALNT2

  • 阳性对照
    • HeLa cell lysate. Human brain tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab102650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50.

靶标

  • 功能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region.
  • 组织特异性Widely expressed.
  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • 结构域There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • 细胞定位Golgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GalNAc T2 antibody
    • GalNAc-T2 antibody
    • Galnt2 antibody
    • GALT2_HUMAN antibody
    • GaNTase 2 antibody
    • Polypeptide GalNAc transferase 2 antibody
    • Polypeptide N acetylgalactosaminyltransferase 2 antibody
    • Polypeptide N acetylgalactosaminyltransferase 2 soluble form antibody
    • Polypeptide N-acetylgalactosaminyltransferase 2 soluble form antibody
    • pp GaNTase 2 antibody
    • pp-GaNTase 2 antibody
    • Protein UDP acetylgalactosaminyltransferase 2 antibody
    • Protein-UDP acetylgalactosaminyltransferase 2 antibody
    • UDP GalNAc transferase 2 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 2 (GalNAc T2) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 2 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 antibody
    see all

Anti-GALNT2 antibody 图像

  • ab102650 staining GALNT2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. A TRITC-conjugated goat anti-rabbit IgG monoclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab102650 staining GALNT2 in formalin fixed Human brain tissue at 1/50 dilution.
  • Anti-GALNT2 antibody (ab102650) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 65 kDa

Anti-GALNT2 antibody (ab102650)参考文献

ab102650 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (HeLa cell)
Specification HeLa cell
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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提交于 Sep 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"