概述

  • 产品名称Anti-GALNT1抗体
    参阅全部 GALNT1 一抗
  • 描述
    小鼠多克隆抗体to GALNT1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human GALNT1 protein (AAH47746.1)

  • 阳性对照
    • GALNT1 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of <10 kDa (predicted molecular weight: Isoform 2, 12 kDa; Isoform 1, 64 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
    • 组织特异性Widely expressed. Expressed in all tissues tested.
    • 通路Protein modification; protein glycosylation.
    • 序列相似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
      Contains 1 ricin B-type lectin domain.
    • 结构域There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
      The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
    • 细胞定位Secreted and Golgi apparatus > Golgi stack membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GALNAC T1 antibody
      • GalNAc transferase 1 antibody
      • GalNAc-T1 antibody
      • GALNT 1 antibody
      • GALNT1 antibody
      • GALT1_HUMAN antibody
      • Polypeptide GalNAc transferase 1 antibody
      • Polypeptide N acetylgalactosaminyltransferase 1 antibody
      • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
      • pp GaNTase 1 antibody
      • pp-GaNTase 1 antibody
      • Protein UDP acetylgalactosaminyltransferase 1 antibody
      • Protein-UDP acetylgalactosaminyltransferase 1 antibody
      • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
      see all

    Anti-GALNT1 antibody 图像

    • All lanes : Anti-GALNT1 antibody (ab69959) at 1/500 dilution

      Lane 1 : GALNT1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 12 kDa
      Observed band size : <10 kDa (why is the actual band size different from the predicted?)

    Anti-GALNT1 antibody (ab69959)参考文献

    ab69959 has not yet been referenced specifically in any publications.

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