Anti-GALNT1抗体(ab109918)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GALNT1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human GALNT1.

  • 阳性对照
    • This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa).

靶标

  • 功能
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
  • 组织特异性
    Widely expressed. Expressed in all tissues tested.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • 结构域
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
  • 细胞定位
    Secreted and Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GALNAC T1 antibody
    • GalNAc transferase 1 antibody
    • GalNAc-T1 antibody
    • GALNT 1 antibody
    • GALNT1 antibody
    • GALT1_HUMAN antibody
    • Polypeptide GalNAc transferase 1 antibody
    • Polypeptide N acetylgalactosaminyltransferase 1 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
    • pp GaNTase 1 antibody
    • pp-GaNTase 1 antibody
    • Protein UDP acetylgalactosaminyltransferase 1 antibody
    • Protein-UDP acetylgalactosaminyltransferase 1 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
    see all

Anti-GALNT1 antibody 图像

  • All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa


    Exposure time : 20 minutes

实验方案

Anti-GALNT1 antibody (ab109918)参考文献

ab109918 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 30 2016

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