概述

  • 产品名称Anti-GALK1抗体
    参阅全部 GALK1 一抗
  • 描述
    兔多克隆抗体to GALK1
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) from the N terminus of GALK1.

  • 阳性对照
    • extracts from HUVEC cells and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab65123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Major enzyme for galactose metabolism.
  • 通路Carbohydrate metabolism; galactose metabolism.
  • 疾病相关Defects in GALK1 are the cause of galactosemia II (GALCT2) [MIM:230200]. Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.
  • 序列相似性Belongs to the GHMP kinase family. GalK subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.6 antibody
    • epididymis secretory protein Li 19 antibody
    • Galactokinase 1 antibody
    • Galactokinase antibody
    • Galactose kinase antibody
    • GALK 1 antibody
    • GALK antibody
    • GALK1 antibody
    • GALK1_HUMAN antibody
    • GK1 antibody
    • GLK antibody
    • GLK1 antibody
    • HEL-S-19 antibody
    see all

Anti-GALK1 antibody 图像

  • All lanes : Anti-GALK1 antibody (ab65123) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HeLa cells
    Lane 3 : extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GALK1 antibody (ab65123)参考文献

ab65123 has not yet been referenced specifically in any publications.

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