概述

  • 产品名称Anti-galectin 9抗体
    参阅全部 galectin 9 一抗
  • 描述
    兔多克隆抗体to galectin 9
  • 特异性This antibody detects endogenous levels of total Galectin-9 protein.
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human galectin 9 aa 51-100 (internal sequence).
    Database link: O00182

  • 阳性对照
    • NIH/3T3 cells. Extracts from HepG2 cells, human lymphoma

性能

应用

Our Abpromise guarantee covers the use of ab69630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • 组织特异性Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • 序列相似性Contains 2 galectin domains.
  • 结构域Contains two homologous but distinct carbohydrate-binding domains.
  • 细胞定位Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 36 kDa beta-galactoside-binding lectin antibody
    • Ecalectin antibody
    • Gal-9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • UAT antibody
    • Urate transporter/channel antibody
    • Urate transporter/channel protein antibody
    see all

Anti-galectin 9 antibody 图像

  • All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa


    Exposure time : 30 seconds
    Ab69630 recognizes the partial tagged recombinant Human galectin 9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
  • ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.

Anti-galectin 9 antibody (ab69630)参考文献

This product has been referenced in:
  • Severson JJ  et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res 3:620-30 (2015). Human . Read more (PubMed: 25701326) »
  • Jiang J  et al. Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer. PLoS One 8:e81799 (2013). IHC-P ; Human . Read more (PubMed: 24339967) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neurons)
Specification Neurons
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Gokhan Yilmaz

Verified customer

提交于 Sep 21 2016

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Please let me know if you have any success by changing the blocking buffer. It is actually not that uncommon to load such amounts of whole cell lysates, however if that is not feasible for your experiments I am...

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Thank you for contacting Abcam regarding ab69630.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to impro...

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We did not test this antibody with a standard protein.This antibody can be used for ELISA due to validation by the following:


1) Peptide-Based ELISA: Coat immunogen peptide in 96-well plate. Det...

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Thank you once again for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As requested, I have obtained a copy of the testing protocol. Please bear in mind that this...

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Thank you for contacting Abcam regarding ab69630.


Regarding our testing conditions for ELISA, this was tested for immunogen-coated ELISA using a conjugated secondary antibody. It has not been tested in sandwich ELISA.


Th...

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Thank you for your enquiry. I have contacted the originator of the product, but unfortunately, there is only limited amount of the peptide and is not available for purchase. I am sorry I can't be of more assistance in this occasion but if there...

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