RabMAb

Anti-Galectin 8抗体[EPR4857] (ab109519)

概述

  • 产品名称Anti-Galectin 8抗体[EPR4857]
    参阅全部 Galectin 8 一抗
  • 描述
    兔单克隆抗体[EPR4857] to Galectin 8
  • 特异性ab109519 has weak cross-reactivity with Galectin 9.
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Galectin 8 aa 1-100. The exact sequence is proprietary.

  • 阳性对照
    • HepG2 or LNCaP cell lysate Human prostatic adenocarcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.

靶标

  • 组织特异性Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
  • 序列相似性Contains 2 galectin domains.
  • 结构域Contains two homologous but distinct carbohydrate-binding domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gal 8 antibody
    • Gal-8 antibody
    • Gal8 antibody
    • Galectin-8 antibody
    • galectin-8g antibody
    • Lectin galactoside binding soluble 8 antibody
    • LEG8_HUMAN antibody
    • LGAL S8 antibody
    • Lgals8 antibody
    • PCTA 1 antibody
    • PCTA-1 antibody
    • PCTA1 antibody
    • Po66 carbohydrate binding protein antibody
    • Po66 carbohydrate-binding protein antibody
    • Po66 CBP antibody
    • Po66-CBP antibody
    • Prostate carcinoma tumor antigen 1 antibody
    see all

Anti-Galectin 8 antibody [EPR4857] 图像

  • ab109519 staining Galectin 8 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab109519 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • ab109519 staining Galectin 8 in the human cell line HEK293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-Galectin 8 antibody [EPR4857] (ab109519) at 1/10000 dilution (purified) + LNCAP (human prostat carcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Blocking and diluting buffer 5% NFDM/TBST
  • All lanes : Anti-Galectin 8 antibody [EPR4857] (ab109519) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : LNCaP cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 40 kDa
  • Lanes 1 & 3 : unpurified at 1/1000 dilution
    Lane 2 : Anti-Galectin 8 antibody [EPR4857] (ab109519) at 1/200 dilution (unpurified)

    Lane 1 : Galectin 9 recombinant protein
    Lane 2 : Galectin 9 recombinant protein
    Lane 3 : Galectin 9 recombinant protein

    Lysates/proteins at 0.02 µg per lane.


    Predicted band size : 40 kDa

    ab109519 has weak cross-reactivity with Galectin 9.

  • Immunohistochemical analysis of paraffin embedded rat kidney tissue section labelling Galectin 8 with purified ab109519 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemical analysis of paraffin embedded mouse kidney tissue section labelling Galectin 8 with purified ab109519 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • ab109519 staining Galectin 8 in human prostatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Unpurified ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
  • Overlay histogram showing HeLa cells stained with unpurified ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109519, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Galectin 8 antibody [EPR4857] (ab109519)参考文献

This product has been referenced in:
  • Copits BA  et al. Modulation of ionotropic glutamate receptor function by vertebrate galectins. J Physiol 592:2079-96 (2014). Rat . Read more (PubMed: 24614744) »
  • Lee YJ  et al. Spatial and temporal expression, and statin responsiveness of galectin-1 and galectin-3 in murine atherosclerosis. Korean Circ J 43:223-30 (2013). IHC-FoFr ; Mouse . Read more (PubMed: 23682281) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (293T, Hela , Raji)
Specification 293T, Hela , Raji
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jun 18 2013

Thank you for contacting us. The peptide used for creating this product maps between amino acids xxx of human Galectin-8 (UniProt: O00214). Unfortunatley we do not offer this for purchase at this time. I hope this information is helpful to yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"