概述
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产品名称Anti-Galectin 8抗体
参阅全部 Galectin 8 一抗 -
描述兔多克隆抗体to Galectin 8
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性
预测可用于: Mouse, Rat, Human, Cynomolgus monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human galectin 8.
(Peptide available as ab42878.)
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阳性对照
- This antibody gave a positive signal in Galectin 8 Tagged Recombinant Protein.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab42879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ICC/IF |
应用说明
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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组织特异性Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
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序列相似性Contains 2 galectin domains.
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结构域Contains two homologous but distinct carbohydrate-binding domains.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 3964 Human
- Entrez Gene: 56048 Mouse
- Entrez Gene: 116641 Rat
- Omim: 606099 Human
- SwissProt: O00214 Human
- SwissProt: Q9JL15 Mouse
- SwissProt: Q62665 Rat
- Unigene: 4082 Human
see all -
别名
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
see all
Anti-Galectin 8 antibody 图像
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Western blot - Galectin 8 antibody (ab42879)Anti-Galectin 8 antibody (ab42879) at 1 µg/ml + Galectin 8 Recombinant Protein at 0.1 µg
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Galectin 8 antibody (ab42879)ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-Galectin 8 antibody (ab42879)参考文献
ab42879 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"