概述

  • 产品名称Anti-Galectin 8抗体
    参阅全部 Galectin 8 一抗
  • 描述
    兔多克隆抗体to Galectin 8
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Human, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human galectin 8.

    (Peptide available as ab42878.)

  • 阳性对照
    • This antibody gave a positive signal in Galectin 8 Tagged Recombinant Protein.

性能

应用

Our Abpromise guarantee covers the use of ab42879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Galectin 8 antibody 图像

    • Anti-Galectin 8 antibody (ab42879) at 1 µg/ml + Galectin 8 Recombinant Protein at 0.1 µg

      Secondary
      Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 35 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    Anti-Galectin 8 antibody (ab42879)参考文献

    ab42879 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"