概述
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产品名称Anti-Galectin 8抗体
参阅全部 Galectin 8 一抗 -
描述兔多克隆抗体to Galectin 8
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性
预测可用于: Mouse, Rat, Human, Cynomolgus monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human galectin 8.
(Peptide available as ab42878.)
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阳性对照
- This antibody gave a positive signal in Galectin 8 Tagged Recombinant Protein.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab42879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ICC/IF |
应用说明
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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组织特异性Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
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序列相似性Contains 2 galectin domains.
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结构域Contains two homologous but distinct carbohydrate-binding domains.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 3964 Human
- Entrez Gene: 56048 Mouse
- Entrez Gene: 116641 Rat
- Omim: 606099 Human
- SwissProt: O00214 Human
- SwissProt: Q9JL15 Mouse
- SwissProt: Q62665 Rat
- Unigene: 4082 Human
see all -
别名
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
see all
图片
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Western blot - Anti-Galectin 8 antibody (ab42879)Anti-Galectin 8 antibody (ab42879) at 1 µg/ml + Galectin 8 Recombinant Protein at 0.1 µg
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
ab42879 recognizes the full length tagged recombinant Galectin 8 protein which has an expected molecular weight of 65.5 kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8 antibody (ab42879)ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
文献
ab42879 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"