重组HRP Anti-Galectin 7抗体[EPR4287] (ab208508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4287] to Galectin 7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
-
产品名称
HRP Anti-Galectin 7抗体[EPR4287]
参阅全部 Galectin 7 一抗 -
描述
HRP兔单克隆抗体[EPR4287] to Galectin 7 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human Skin tissue lysate. IHC-P: FFPE human cervix (normal) tissue sections.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4287 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208508于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
|
说明 |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). |
靶标
-
功能
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. -
组织特异性
Mainly expressed in stratified squamous epithelium. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
-
数据库链接
- Entrez Gene: 3963 Human
- Entrez Gene: 653499 Human
- Omim: 600615 Human
- SwissProt: P47929 Human
- Unigene: 558355 Human
- Unigene: 707031 Human
-
别名
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
see all
图片
-
IHC image of Galectin 7 staining in a section of formalin-fixed paraffin-embedded normal human cervix*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208508, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
HRP Anti-Galectin 7 antibody [EPR4287] (ab208508) at 1/1000 dilution + Human skin tissue lysate - total protein (ab30166) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208508 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab208508 尚未被引用在任何文献中。