Anti-Galectin 7抗体(ab10482)
Key features and details
- Rabbit polyclonal to Galectin 7
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Galectin 7抗体
参阅全部 Galectin 7 一抗 -
描述
兔多克隆抗体to Galectin 7 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Galectin 7 aa 100 to the C-terminus.
Database link: 3963 -
常规说明
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody说明
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10482于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
|
ICC/IF | (2) |
Use at an assay dependent concentration.
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IP |
Use at 10-20 µg/mg of lysate.
|
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IHC-P |
Use at an assay dependent concentration.
|
说明 |
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WB
1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at 10-20 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. -
组织特异性
Mainly expressed in stratified squamous epithelium. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 3963 Human
- Entrez Gene: 653499 Human
- Entrez Gene: 16858 Mouse
- Omim: 600615 Human
- SwissProt: P47929 Human
- SwissProt: O54974 Mouse
- Unigene: 558355 Human
- Unigene: 707031 Human
see all -
别名
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
see all
图片
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ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10
ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10 µg) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20 µg) from the epidermis of FVB wild-type mice. Antibody: ab10482 used at 0.005 (A) or 0.0025 (B) µg/ml for WB and as indicated forµ g) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20µ g) from the epidermis of FVB wild-type mice. Antibody: ab10482 used at 0.005 (A) or 0.0025 (B)µ g/ml for WB and as indicated for IP. Detection: Chemiluminescence with 1 second exposure time.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab10482 被引用在 9 文献中.
- Umayahara T et al. Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis. Clin Exp Allergy N/A:N/A (2020). PubMed: 32474952
- Trebo A et al. High Galectin-7 and Low Galectin-8 Expression and the Combination of both are Negative Prognosticators for Breast Cancer Patients. Cancers (Basel) 12:N/A (2020). PubMed: 32290551
- Lin CY et al. Autophagy Receptor Tollip Facilitates Bacterial Autophagy by Recruiting Galectin-7 in Response to Group A Streptococcus Infection. Front Cell Infect Microbiol 10:583137 (2020). PubMed: 33425778
- Lionnard L et al. TRIM17 and TRIM28 antagonistically regulate the ubiquitination and anti-apoptotic activity of BCL2A1. Cell Death Differ N/A:N/A (2018). PubMed: 30042493
- Advedissian T et al. E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface. Sci Rep 7:17086 (2017). PubMed: 29213102
- Monari E et al. Analysis of protein expression in periodontal pocket tissue: a preliminary study. Proteome Sci 13:33 (2015). WB . PubMed: 26719749
- Gendronneau G et al. Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair. PLoS One 10:e0119031 (2015). PubMed: 25741714
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492
- Honoré B et al. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci 6:18 (2008). WB . PubMed: 18544163