使用敲除细胞株进行验证

Anti-Galectin 3抗体[RM0082-5L36] (ab86058)

概述

  • 产品名称
    Anti-Galectin 3抗体[RM0082-5L36]
    参阅全部 Galectin 3 一抗
  • 描述
    大鼠单克隆抗体[RM0082-5L36] to Galectin 3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length Mouse protein

性能

应用

Our Abpromise guarantee covers the use of ab86058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.

靶标

  • 功能
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 组织特异性
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 序列相似性
    Contains 1 galectin domain.
  • 细胞定位
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

图片



  • Predicted band size : 26 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Galectin 3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab86058 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab86058 was shown to recognize Galectin 3 when Galectin 3 (KO) knockout samples were used, along with additional cross-reactive bands. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE. ab86058 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Galectin 3 antibody [RM0082-5L36] (ab86058) + Lysates of mouse 4T1 cells

    Predicted band size : 26 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab86058 has not yet been referenced specifically in any publications.

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