RabMAb

Anti-Galectin 3抗体[EPR2774] (ab76466)

概述

  • 产品名称Anti-Galectin 3抗体[EPR2774]
    参阅全部 Galectin 3 一抗
  • 描述
    兔单克隆抗体[EPR2774] to Galectin 3
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in human Galectin 3

  • 阳性对照
    • WB: HeLa, SW480, A431 and MCF7 cell lysates ICC/IF: HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/30 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
    • 组织特异性A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
    • 序列相似性Contains 1 galectin domain.
    • 细胞定位Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    Anti-Galectin 3 antibody [EPR2774] 图像

    • Overlay histogram showing THP1 cells stained with ab76466 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76466, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab76466, at a 1/100 dilution, staining Galectin 3 in HeLa cells by Immunofluorescence.
    • All lanes : Anti-Galectin 3 antibody [EPR2774] (ab76466) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 26 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Anti-Galectin 3 antibody [EPR2774] (ab76466)参考文献

    ab76466 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (BMDM)
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris gel, MES running buffer)
    Loading amount 5 µg
    Specification BMDM
    Blocking step Licor TBS blocking buffer as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 22°C
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    提交于 Aug 24 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"