概述

  • 产品名称
    Anti-Galectin 3抗体
    参阅全部 Galectin 3 一抗
  • 描述
    兔多克隆抗体to Galectin 3
  • 特异性
    Galectin 3 antibody detects endogenous levels of total Galectin 3 protein.
  • 经测试应用
    适用于: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Galectin 3 (internal sequence). The immunogen is in the range of aa 141-190.
    Database link: P17931

  • 阳性对照
    • human lung carcinoma tissue; Hela cell extract

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 21652631
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 组织特异性
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 序列相似性
    Contains 1 galectin domain.
  • 细胞定位
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).
  • All lanes : Anti-Galectin 3 antibody (ab53082) at 1/500 dilution

    Lane 1 : Hela cell extract with no blocking peptide
    Lane 2 : Hela cell extract with blocking peptide (ab53082 immunogen)


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunocytochemical analysis of mouse macrophage cells, labeling Galectin 3 with ab53082. Cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked in 1% BSA for 30 minutes at 37°C. Incubation with ab53082 (diluted 1/500 in PBS) was for 1 hour at 37°C. The secondary antibody used was ab150073 diluted 1/500.

    See Abreview

文献

This product has been referenced in:
  • ElMokh O  et al. Combined MEK and Pi3'-kinase inhibition reveals synergy in targeting thyroid cancer in vitro and in vivo. Oncotarget 8:24604-24620 (2017). IHC-P ; Mouse . Read more (PubMed: 28445948) »
  • Vidal PM  et al. Delayed decompression exacerbates ischemia-reperfusion injury in cervical compressive myelopathy. JCI Insight 2:N/A (2017). Read more (PubMed: 28570271) »

See all 12 Publications for this product

客户评价及客户问答

Abreviews
Application
Blocking
Sample
Human Cell (colorectal carcinoma)
Specification
colorectal carcinoma
Username

Abcam user community

Verified customer

提交于 Oct 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Mouse Cell (Macrophage)
Specification
Macrophage
Permeabilization
Yes - Tx100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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