概述

  • 产品名称Anti-Galectin 3抗体
    参阅全部 Galectin 3 一抗
  • 描述
    兔多克隆抗体to Galectin 3
  • 特异性Galectin 3 antibody detects endogenous levels of total Galectin 3 protein.
  • 经测试应用适用于: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Galectin 3 (internal sequence). The immunogen is in the range of aa 141-190.
    Database link: P17931

  • 阳性对照
    • human lung carcinoma tissue; Hela cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 21652631
ICC/IF 1/100 - 1/500.

靶标

  • 功能Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 组织特异性A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 序列相似性Contains 1 galectin domain.
  • 细胞定位Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Anti-Galectin 3 antibody 图像

  • All lanes : Anti-Galectin 3 antibody (ab53082) at 1/500 dilution

    Lane 1 : Hela cell extract with no blocking peptide
    Lane 2 : Hela cell extract with blocking peptide (ab53082 immunogen)


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).

Anti-Galectin 3 antibody (ab53082)参考文献

This product has been referenced in:
  • Volarevic V  et al. Gal-3 regulates the capacity of dendritic cells to promote NKT-cell-induced liver injury. Eur J Immunol 45:531-43 (2015). Read more (PubMed: 25359399) »
  • Fry JL  et al. Vascular Smooth Muscle Sirtuin-1 Protects Against Aortic Dissection During Angiotensin II-Induced Hypertension. J Am Heart Assoc 4:e002384 (2015). WB ; Mouse . Read more (PubMed: 26376991) »

See all 7 Publications for this product

Product Wall

Abreviews
Application Blocking
Sample Human Cell (colorectal carcinoma)
Specification colorectal carcinoma
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Verified customer

提交于 Oct 27 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Mouse Cell (Macrophage)
Specification Macrophage
Permeabilization Yes - Tx100
Fixative Formaldehyde
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提交于 Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"