- 数据表
- 具体的参考文献 (6)
- 实验方案
概述
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产品名称Anti-Galectin 3抗体
参阅全部 Galectin 3 一抗 -
描述兔多克隆抗体to Galectin 3
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经测试应用适用于: ICC/IF, WBmore details
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种属反应性与反应: Human
预测可用于: Dog, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Galectin 3.
(Peptide available as ab31706.)
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阳性对照
- HeLa (Human epithelial carcinoma cell line) Whole Cell and Nuclear Lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab31707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
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组织特异性A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
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序列相似性Contains 1 galectin domain.
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细胞定位Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
- Information by UniProt
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数据库链接
- Entrez Gene: 3958 Human
- Omim: 153619 Human
- SwissProt: P38486 Dog
- SwissProt: P17931 Human
- Unigene: 531081 Human
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别名
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
see all
Anti-Galectin 3 antibody 图像
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Western blot - Anti-Galectin 3 antibody (ab31707)
Predicted band size : 26 kDaLane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Galectin 3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab31707 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab31707 was shown to recognize Galectin 3 when Galectin 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE. Ab31707 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Galectin 3 antibody (ab31707)ICC/IF image of ab31707 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31707, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-Galectin 3 antibody (ab31707)All lanes : Anti-Galectin 3 antibody (ab31707) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
We also see a very faint band at 52 kDa in the HeLa Whole cell lysate, we are uncertain as to the identity of this band.
Anti-Galectin 3 antibody (ab31707)参考文献
This product has been referenced in:
- Truchan HK et al. Type II Secretion Substrates of Legionella pneumophila Translocate Out of the Pathogen-Occupied Vacuole via a Semipermeable Membrane. MBio 8:N/A (2017). ICC/IF . Read more (PubMed: 28634242) »
- Coppin L et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017). Read more (PubMed: 28262838) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"