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RabMAb

Anti-Galectin 1抗体[EPR3205] (ab108389)

概述

  • 产品名称
    Anti-Galectin 1抗体[EPR3205]
    参阅全部 Galectin 1 一抗
  • 描述
    兔单克隆抗体[EPR3205] to Galectin 1
  • 经测试应用
    适用于: WB, IP, ICC/IF, IHC-FoFr, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Galectin 1

  • 阳性对照
    • HL60 cell lysate, K562 cell lysate, NIH 3T3 cell lysate, mouse heart tissue lysate, mouse kidney cell lysate, mouse spleen cell lysate, rat heart tissue lysate, rat kidney tissue lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab108389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23682281
Flow Cyt 1/100.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
    • 组织特异性
      Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
    • 序列相似性
      Contains 1 galectin domain.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14 kDa laminin-binding protein antibody
      • 14 kDa lectin antibody
      • Beta galactoside binding lectin antibody
      • Beta galactoside binding lectin L 14 I antibody
      • beta galactoside binding protein antibody
      • Beta-galactoside-binding lectin L-14-I antibody
      • DKFZp686E23103 antibody
      • Gal 1 antibody
      • Gal-1 antibody
      • GAL1 antibody
      • Galaptin antibody
      • Galbp antibody
      • Galectin antibody
      • Galectin-1 antibody
      • Galectin1 antibody
      • GBP antibody
      • HBL antibody
      • HLBP14 antibody
      • HPL antibody
      • L 14.5 antibody
      • L-14.5 antibody
      • L14 antibody
      • Lactose binding lectin 1 antibody
      • Lactose-binding lectin 1 antibody
      • Lect14 antibody
      • Lectin galactoside binding soluble 1 antibody
      • Lectin galactoside-binding soluble 1 antibody
      • LEG1_HUMAN antibody
      • LGALS 1 antibody
      • LGALS1 antibody
      • Lgals1 lectin galactose binding soluble 1 antibody
      • MAPK activating protein MP12 antibody
      • Putative MAPK activating protein MP12 antibody
      • Putative MAPK-activating protein PM12 antibody
      • S Lac lectin 1 antibody
      • S-Lac lectin 1 antibody
      see all

    Anti-Galectin 1 antibody [EPR3205] 图像

    • ab108389 staining Galectin 1 in NIH/3T3(mouse embryo) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

      Lane 1 : mouse heart tissue lysate
      Lane 2 : mouse kidney tissue lysate
      Lane 3 : mouse spleen tissue lysate
      Lane 4 : rat heart tissue lysate
      Lane 5 : rat kidney tissue lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa

    Anti-Galectin 1 antibody [EPR3205] (ab108389)参考文献

    This product has been referenced in:
    • Pereira PM  et al. Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLoS One 9:e95529 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24763311) »
    • Gurel Z  et al. Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy. PLoS One 9:e95561 (2014). WB ; Mouse . Read more (PubMed: 24788674) »

    See all 4 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Treatment
    alpha synuclein 3hr
    Specification
    human
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 30 2015



    As this is an Epitomics product, I have forwarded your request to them. They will be in catact with you shortly.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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