概述

  • 产品名称Anti-Galanin抗体
    参阅全部 Galanin 一抗
  • 描述
    兔多克隆抗体to Galanin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Galanin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P22466

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa).

靶标

Anti-Galanin antibody 图像

  • Anti-Galanin antibody (ab87378) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes

    The predicted molecular weight of Galanin is 13 kDa (SwissProt), however we expect to observe a banding pattern at 16 kDa.

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab87378 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-Galanin antibody (ab87378)参考文献

ab87378 has not yet been referenced specifically in any publications.

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