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The predicted molecular weight of Galanin is 13 kDa (SwissProt), however we expect to observe a banding pattern at 16 kDa.
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab87378 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Anti-Galanin antibody (ab87378)参考文献
has not yet been referenced specifically in any publications.