概述
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产品名称Anti-Galanin抗体
参阅全部 Galanin 一抗 -
描述兔多克隆抗体to Galanin
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Chicken, Pig, Chimpanzee, Macaque monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human Galanin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P22466 -
阳性对照
- This antibody gave a positive signal in Human Pancreas tissue lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab87378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa). |
靶标
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功能Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion.
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序列相似性Belongs to the galanin family.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 423117 Chicken
- Entrez Gene: 51083 Human
- Entrez Gene: 14419 Mouse
- Entrez Gene: 397465 Pig
- Entrez Gene: 29141 Rat
- Omim: 137035 Human
- SwissProt: P30802 Chicken
- SwissProt: P22466 Human
see all -
别名
- GAL 1 antibody
- GAL antibody
- GAL GMAP antibody
see all
图片
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Western blot - Anti-Galanin antibody (ab87378)Anti-Galanin antibody (ab87378) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 16 kDa (why is the actual band size different from the predicted?)
Exposure time: 16 minutesThe predicted molecular weight of Galanin is 13 kDa (SwissProt), however we expect to observe a banding pattern at 16 kDa.
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab87378 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
文献
ab87378 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"