Synthetic peptide corresponding to Human Galanin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P22466
Our Abpromise guarantee covers the use of ab87378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa). |
The predicted molecular weight of Galanin is 13 kDa (SwissProt), however we expect to observe a banding pattern at 16 kDa.
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab87378 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab87378 has not yet been referenced specifically in any publications.
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