Anti-GAL4抗体[3G152] (ab14477)

概述

  • 产品名称Anti-GAL4抗体[3G152]
    参阅全部 GAL4 一抗
  • 描述
    小鼠单克隆抗体[3G152] to GAL4
  • 特异性This antibody can be used to detect both N-terminal and C-terminal tagged Gal4(1–147) and Gal4(1–92) fusion proteins.
  • 经测试应用适用于: ICC/IF, ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-147 of S. cerevisiae GAL4.

  • 表位The immunoreactive epitope maps within amino acids 1–92 of GAL4.
  • 阳性对照
    • N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins

性能

应用

Our Abpromise guarantee covers the use of ab14477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20610754
ELISA Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 3 µg/ml. Predicted molecular weight: 99 kDa.
ICC Use at an assay dependent concentration.

靶标

  • 相关性Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
  • 细胞定位Nuclear
  • 数据库链接
    • 别名
      • Gal4p antibody
      • GAL81 antibody
      • Regulatory protein GAL4 antibody

    Anti-GAL4 antibody [3G152] (ab14477)参考文献

    This product has been referenced in:
    • Chen Y  et al. Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons. J Neurosci 30:9199-208 (2010). ICC/IF . Read more (PubMed: 20610754) »

    See 1 Publication for this product

    Product Wall

    Thisantibody has only been tested with GAL4-fusion proteins inWestern Blot.

    I hope this is helpful. Please contact me again if you have any further questions.

    Application Immunocytochemistry
    Sample Human Recombinant protein (Transfected cells)
    Specification Transfected cells
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
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    Verified customer

    提交于 Aug 17 2005

    Thank you for contacting us for technical support and taking the time to fill in our protocol questionnaire, I'm very sorry to hear you are experiencing problems with ab14477 in your samples. I have looked at your order details and can confirm ther...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"