产品名称 Anti-GAK protein抗体
兔多克隆抗体to GAK protein
WB, ELISA, IHC-P more details
Synthetic peptide corresponding to Human GAK aa 15-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q6P490
Mouse heart tissue lysate. Human hepatocarcinoma tissue.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Concentration information loading...
纯度 Protein G purified
纯化说明 This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 62 kDa.
1/50 - 1/100.
相关性 GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.
(Information taken form the supplier datasheet for ab86397)
Anti-GAK protein antibody 图像
Western blot - GAK antibody - N-terminal (ab86397)
Anti-GAK protein antibody (ab86397) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg Predicted band size : 62 kDa Observed band size : 62 kDa Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAK antibody - N-terminal (ab86397)
ab86397 at a 1/1000 dilution staining GAK in hepatocarcinoma tissue. DAB staining.
Anti-GAK protein antibody (ab86397)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"