概述

  • 产品名称Anti-GAG抗体
  • 描述
    兔多克隆抗体to GAG
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    Recombinant Murine Leukemia Virus GAG p30 capsid protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab100970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1500 - 1/2000. Predicted molecular weight: 30 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性Murine leukemia viruses belong to family of Retroviruses group and are named for their ability to cause cancer in mice. Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important roles at several stages in the viral life cycle. Late in infection, the Gag precursor protein acts to mediate the assembly and release of virion particles. After budding, the core matures by cleavage of Gag precursor protein into the mature core proteins, the matrix (MA), capsid (CA) and nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of infection.

Anti-GAG antibody 图像

  • All lanes : Anti-GAG antibody (ab100970) at 1/1500 dilution

    Lane 1 : PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas);
    Lane 2 : HEK293 cells
    Lane 3 : GAG protein expressed in HEK293 cells
    Lane 4 : PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas);
    Lane 5 : CHO cells
    Lane 6 : Gag protein expressed in CHO cells

    Secondary
    Alkaline phosphatase conjugated anti rabbit IgG at 1/5000 dilution

    Predicted band size : 30 kDa

Anti-GAG antibody (ab100970)参考文献

This product has been referenced in:
  • McNally DJ  et al. Optimised concentration and purification of retroviruses using membrane chromatography. J Chromatogr A 1340:24-32 (2014). WB ; Mouse . Read more (PubMed: 24685165) »
  • Yin D  et al. Convection-enhanced delivery improves distribution and efficacy of tumor-selective retroviral replicating vectors in a rodent brain tumor model. Cancer Gene Ther 20:336-41 (2013). Read more (PubMed: 23703472) »

See all 2 Publications for this product

Product Wall


The antibody ab100970 has been tested in Western Blot with cell lysates that contain MLV-Gag polyprotein (expressed either in HEK or CHO cells).
The MLV-Gag polyprotein consist of four different proteins:
· Matrix protein p15
&m...

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The antibody A1-910-100 has been tested in Western Blot with cell lysates that contain MLV-Gag polyprotein (expressed either in HEK or CHO cells).
The MLV-Gag polyprotein consist of four different proteins:
· Matrix protein p15
· RNA-b...

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Your protocol seems reasonable, but the antigen retrieval probably does not need to be for a full hour. The lengthy incubation in the hot ER2 may in fact be causing the high background, if the section is lifting off the slide, or if the retrieval is ov...

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Thank you for the protocol details. The GAG antibody has not been tested previously for IHC, at least not to our knowledge, so I do not have suggestions based on testing of this antibody. In general, you will want to ensure that you include a blocking ...

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Thank you for contacting us.

We have not tested this antibody in IHC, but I may be able to offer some general advice. Can you please tell me a little about your samples, how they are prepared, and how they are stained? For instance, what is ...

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Thank you for contacting us.

Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab100970.

However, ab10097...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"