The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
The transcriptional regulator gadE regulates the expression of several genes involved in acid resistance, including gadA and gadBC, regardless of media or growth conditions. It binds directly to the 20 bp GAD box found in various gene control regions. gadE could be involved in the regulation of the genes coding for the type III secretion system in enterohaemorragic strains. It contains 1 HTH luxR-type DNA-binding domain.