Anti-GADD45B抗体(ab105060)

概述

  • 产品名称Anti-GADD45B抗体
    参阅全部 GADD45B 一抗
  • 描述
    兔多克隆抗体to GADD45B
  • 经测试应用适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 81-130 (FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW) of Human GADD45B (NP_056490).Peptide available as ab108448.

  • 阳性对照
    • HepG2 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung. IF/ICC: PANC-1 cell line

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab105060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 18 kDa.Can be blocked with GADD45B peptide (ab108448). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
  • 序列相似性Belongs to the GADD45 family.
  • Information by UniProt
  • 数据库链接
  • 形式It localizes to the nucleus.
  • 别名
    • GA45B_HUMAN antibody
    • GADD45 Beta antibody
    • Gadd45b antibody
    • GADD45BETA antibody
    • Growth arrest and DNA damage inducible 45 beta antibody
    • Growth arrest and DNA damage inducible beta antibody
    • Growth arrest and DNA damage-inducible gene GADD45, beta antibody
    • Growth arrest and DNA damage-inducible protein GADD45 beta antibody
    • Growth arrest and DNA-damage-inducible protein GADD45 beta antibody
    • MY118 antibody
    • MYD118 antibody
    • MYD118, mouse, homolog of antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD118 antibody
    • Negative growth regulatory protein MyD118 antibody
    • Negative growth-regulatory protein MyD118 antibody
    see all

Anti-GADD45B antibody 图像

  • Anti-GADD45B antibody (ab105060) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 18 kDa
  • Anti-GADD45B antibody (ab105060) at 1 µg/ml + Recombinant Human GADD45B protein (ab79246) at 1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds
  • IHC image of GADD45B staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105060, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab105060 stained PANC-1 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105060, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • GADD45B was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to GADD45B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab105060.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 17kDa; non specific bands - 34 and 76kDa: We are unsure as to the identity of this extra band; GADD45B

Anti-GADD45B antibody (ab105060)参考文献

ab105060 has not yet been referenced specifically in any publications.

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