Anti-GADD45A抗体- Aminoterminal end (ab76664)

概述

  • 产品名称Anti-GADD45A抗体- Aminoterminal end
    参阅全部 GADD45A 一抗
  • 描述
    兔多克隆抗体to GADD45A - Aminoterminal end
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N terminal region of human GADD45A.

  • 阳性对照
    • Y79 cell line lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab76664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 23706118
WB 1/50 - 1/100. Predicted molecular weight: 18 kDa.
ELISA 1/1000.

靶标

  • 功能Binds to proliferating cell nuclear antigen. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
  • 序列相似性Belongs to the GADD45 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DDIT 1 antibody
    • DDIT-1 antibody
    • DDIT1 antibody
    • DNA damage inducible transcript 1 antibody
    • DNA damage-inducible transcript 1 protein antibody
    • GA45A_HUMAN antibody
    • GADD45 antibody
    • GADD45A antibody
    • Growth arrest and DNA damage inducible 45 alpha antibody
    • Growth arrest and DNA damage inducible alpha antibody
    • Growth arrest and DNA damage-inducible protein GADD45 alpha antibody
    see all

Anti-GADD45A antibody - Aminoterminal end 图像

  • Anti-GADD45A antibody - Aminoterminal end (ab76664) at 1/1000 dilution + Y79 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 18 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-GADD45A antibody - Aminoterminal end (ab76664)参考文献

This product has been referenced in:
  • Spassov S  et al. Genetic targets of hydrogen sulfide in ventilator-induced lung injury--a microarray study. PLoS One 9:e102401 (2014). WB ; Mouse . Read more (PubMed: 25025333) »
  • Tront JS  et al. Gadd45a levels in human breast cancer are hormone receptor dependent. J Transl Med 11:131 (2013). IHC-P ; Human . Read more (PubMed: 23706118) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human cell line CHRF)
Total protein in input 1000 µg
Immuno-precipitation step Protein A/G
Specification human cell line CHRF
Username

Abcam user community

Verified customer

提交于 Jun 26 2015

Thank you for contacting us.

We do not have a Gadd45a antibody that has been tested for IP. I was going to recommend ab76664 as the best option, assuming the IHC review that a customer sent was positive. (Positive IHC results suggest an anti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast Cancer)
Specification Breast Cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

提交于 Dec 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"