概述

  • 产品名称Anti-GADD34抗体
    参阅全部 GADD34 一抗
  • 描述
    山羊多克隆抗体to GADD34
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide: AAALDLSGRRG., corresponding to C terminal amino acids 664-674 of Human GADD34.

  • 阳性对照
    • U937 lysate
  • 常规说明GenBank Accession Number – NP_055145 LocusLink ID - 23645

性能

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应用

Our Abpromise guarantee covers the use of ab9869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human GADD34 peptide (ab23166).

靶标

  • 功能Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
  • 序列相似性Belongs to the PPP1R15 family.
  • 翻译后修饰Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
  • 细胞定位Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis associated protein antibody
    • GADD 34 antibody
    • Growth arrest and DNA damage inducible 34 antibody
    • Growth arrest and DNA damage-inducible protein GADD34 antibody
    • Myeloid differentiation primary response protein MyD116 homolog antibody
    • Ppp1r15a antibody
    • PR15A_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody
    • Protein phosphatase 1 regulatory subunit 15A antibody
    see all

Anti-GADD34 antibody 图像

  • Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg

    Predicted band size : 75 kDa
  • IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GADD34 antibody (ab9869)参考文献

This product has been referenced in:
  • Galindo I  et al. The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection. Cell Death Dis 3:e341 (2012). WB . Read more (PubMed: 22764100) »
  • Panaretakis T  et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB ; Mouse . Read more (PubMed: 19165151) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jun 19 2007

Many thanks for you enquiry regarding GADD34 antibody. We aren't very sure whether we understood the question correctly. If its a problem with sensitivity, on the cell lysate we tested (U937)mentioned in the datsheet, we repeatedly achieved the signal....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"