- 数据表
- 具体的参考文献 (26)
- 实验方案
概述
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产品名称Anti-GAD67抗体[K-87]
参阅全部 GAD67 一抗 -
描述小鼠单克隆抗体[K-87] to GAD67
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经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, WB, IHC-Frmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide:
RFRRTETDFSNLFARDLLPA
, corresponding to amino acids 87-106 of Human GAD67 -
阳性对照
- In Western Blot, this antibody gave a positive signal in Mouse and Rat Brain Tissue Lysates. GAD67 has been thought to be primarily located in the nerve cell body, but using this new K-87 mAb, GAD67 can now also be detected in dendrites and axons.
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常规说明
ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... -
纯度Protein A purified
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Primary antibody说明ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).
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克隆单克隆
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克隆编号K-87
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同种型IgG1
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轻链类型kappa
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab26116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| Flow Cyt | Use 1µg for 106 cells. (methanol fixed cells) |
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| IHC-P | 1/1000 - 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998). | |
| WB | 1/1000 - 1/20000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). | |
| IHC-Fr | 1/2000. |
靶标
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功能Catalyzes the production of GABA.
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组织特异性Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
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疾病相关Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
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序列相似性Belongs to the group II decarboxylase family.
- Information by UniProt
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数据库链接
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 24379 Rat
- Omim: 605363 Human
- SwissProt: Q99259 Human
- SwissProt: P48318 Mouse
- SwissProt: P18088 Rat
- Unigene: 420036 Human
see all -
别名
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
Anti-GAD67 antibody [K-87] 图像
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![Western blot - Anti-GAD67 antibody [K-87] (ab26116)](http://www.abcam.cn/ps/products/26/ab26116/Images/ab26116-244254-26116-WB.jpg)
Western blot - Anti-GAD67 antibody [K-87] (ab26116)Anti-GAD67 antibody [K-87] (ab26116) at 1/2000 dilution + mouse cerebellum at 0.75 µg
Secondary
HRP horse anti-mouse IgG at 1/5000 dilution
Predicted band size : 67 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [K-87] (ab26116)](http://www.abcam.cn/ps/products/26/ab26116/Images/ab26116_1.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [K-87] (ab26116)ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [K-87] (ab26116)](http://www.abcam.cn/ps/products/26/ab26116/Images/ab26116-244224-26116IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [K-87] (ab26116)Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.
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![Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] (ab26116)](http://www.abcam.cn/ps/products/26/ab26116/Images/GAD67-Primary-antibodies-ab26116-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] (ab26116)ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-GAD67 antibody [K-87] (ab26116)](http://www.abcam.cn/ps/products/26/ab26116/Images/GAD67-Primary-antibodies-ab26116-3.jpg)
Flow Cytometry - Anti-GAD67 antibody [K-87] (ab26116)Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
Anti-GAD67 antibody [K-87] (ab26116)参考文献
This product has been referenced in:
- Umorin M et al. Genes in the GABA Pathway Increase in the Lateral Thalamus of Sprague-Dawley Rats During the Proestrus/Estrus Phase. J Cell Physiol 231:1057-64 (2016). Read more (PubMed: 26388520) »
- Cheng YC et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). ICC/IF ; Human . Read more (PubMed: 27444754) »
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