Anti-GAD67/GAD1抗体(ab97739)
Key features and details
- Rabbit polyclonal to GAD67/GAD1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GAD67/GAD1抗体
参阅全部 GAD67/GAD1 一抗 -
描述
兔多克隆抗体to GAD67/GAD1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Cat, Dog, Pig, Zebrafish -
免疫原
Recombinant fragment within Human GAD1/GAD67 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q99259-1 -
阳性对照
- H1299, HeLa, HepG2, Molt-4, Raji and NIH-3T3 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97739于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
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ICC/IF | (1) |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Catalyzes the production of GABA. -
组织特异性
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
疾病相关
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
序列相似性
Belongs to the group II decarboxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 493699 Cat
- Entrez Gene: 517552 Cow
- Entrez Gene: 478794 Dog
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 24379 Rat
- Entrez Gene: 378441 Zebrafish
see all -
别名
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
图片
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Anti-GAD67/GAD1 antibody (ab97739) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Anti-GAD67/GAD1 antibody (ab97739) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GAD1/GAD67 antibody (ab97739) at 1/200 dilution. Lower image was also merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab97739 被引用在 10 文献中.
- Ji NN et al. Microglia-derived TNF-α inhibiting GABAergic neurons in the anterior lateral bed nucleus of the stria terminalis precipitates visceral hypersensitivity induced by colorectal distension in rats. Neurobiol Stress 18:100449 (2022). PubMed: 35521065
- Krishnan V et al. Peripheral Nerve Injury Induces Changes in the Activity of Inhibitory Interneurons as Visualized in Transgenic GAD1-GCaMP6s Rats. Biosensors (Basel) 12:N/A (2022). PubMed: 35735531
- Chen X et al. Entorhinal cortex-based metabolic profiling of chronic restraint stress mice model of depression. Aging (Albany NY) 12:3042-3052 (2020). PubMed: 32074509
- Zhao X et al. Anodal and cathodal tDCS modulate neural activity and selectively affect GABA and glutamate syntheses in the visual cortex of cats. J Physiol 598:3727-3745 (2020). PubMed: 32506434
- Nobili A et al. Ambra1 Shapes Hippocampal Inhibition/Excitation Balance: Role in Neurodevelopmental Disorders. Mol Neurobiol 55:7921-7940 (2018). PubMed: 29488136
- Ding Y et al. Changes in GABAergic markers accompany degradation of neuronal function in the primary visual cortex of senescent rats. Sci Rep 7:14897 (2017). WB . PubMed: 29097694
- Casalia ML et al. Persistent seizure control in epileptic mice transplanted with gamma-aminobutyric acid progenitors. Ann Neurol 82:530-542 (2017). PubMed: 28833459
- Moraga A et al. Aging increases microglial proliferation, delays cell migration, and decreases cortical neurogenesis after focal cerebral ischemia. J Neuroinflammation 12:87 (2015). IF ; Mouse . PubMed: 25958332
- Stansfield KH et al. Early-life lead exposure recapitulates the selective loss of parvalbumin-positive GABAergic interneurons and subcortical dopamine system hyperactivity present in schizophrenia. Transl Psychiatry 5:e522 (2015). WB . PubMed: 25756805
- Bajo VM et al. The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-40 (2014). IHC ; Ferret . PubMed: 24945075