概述

  • 产品名称
  • 描述
    山羊多克隆抗体to GAD67
  • 宿主
    Goat
  • 特异性
    ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Cat, Dog, Pig, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide:

    C-PDSPQRREKLHK

    , corresponding to internal sequence amino acids 526 - 537 of Human GAD67 (NP_000808.2)

  • 阳性对照
    • Mouse brain lysate, Rat brain lysate and Human brain (cerebellum) lysate. IHC-P: Human hippocampus.

性能

应用

Our Abpromise guarantee covers the use of ab80589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • IHC image of GAD67 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80589, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-GAD67 antibody (ab80589) at 1 µg/ml + Mouse Brain lysate (in RIPA buffer) at 35 µg

    Predicted band size: 67 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • Anti-GAD67 antibody (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

文献

This product has been referenced in:
  • Taylor SR  et al. GABAergic and glutamatergic efferents of the mouse ventral tegmental area. J Comp Neurol 522:3308-34 (2014). IHC-FrFl ; Mouse . Read more (PubMed: 24715505) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab80589.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册