山羊多克隆抗体to GAD67/GAD1 (ab80589)
Key features and details
- Goat polyclonal to GAD67/GAD1
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
山羊多克隆抗体to GAD67/GAD1
参阅全部 GAD67/GAD1 一抗 -
宿主
Goat -
特异性
ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65). -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
免疫原
Synthetic peptide corresponding to Human GAD1/GAD67 aa 500 to the C-terminus (internal sequence) (Cysteine residue). NP_000808.2.
Database link: Q99259-1 -
阳性对照
- WB: Human brain (cerebral cortex) lysate in RIPA buffer. Flow Cyt: A431 cells; ICC/IF: A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab80589 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80589于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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Flow Cyt
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Catalyzes the production of GABA. -
组织特异性
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
疾病相关
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
序列相似性
Belongs to the group II decarboxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 493699 Cat
- Entrez Gene: 468557 Chimpanzee
- Entrez Gene: 517552 Cow
- Entrez Gene: 478794 Dog
- Entrez Gene: 2571 Human
- Entrez Gene: 100173252 Orangutan
- Entrez Gene: 396928 Pig
- Omim: 605363 Human
see all -
别名
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
图片
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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Goat polyclonal to GAD67/GAD1 (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 67 kDa -
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
数据表及文件
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Datasheet download
文献 (5)
ab80589 被引用在 5 文献中.
- Yao H et al. Methadone interrupts neural growth and function in human cortical organoids. Stem Cell Res 49:102065 (2020). PubMed: 33137567
- Ellis JK et al. Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams. J Comp Neurol N/A:N/A (2019). PubMed: 31050805
- Corbridge SM et al. JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy. J Neurovirol N/A:N/A (2019). PubMed: 31025264
- Zhuang QX et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest 128:5413-5427 (2018). PubMed: 30226827
- Taylor SR et al. GABAergic and glutamatergic efferents of the mouse ventral tegmental area. J Comp Neurol 522:3308-34 (2014). IHC-FrFl ; Mouse . PubMed: 24715505