Anti-GAD67抗体(ab104561)

概述

  • 产品名称Anti-GAD67抗体
    参阅全部 GAD67 一抗
  • 描述
    山羊多克隆抗体to GAD67
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-PDSPQRREKLHK

    , corresponding to internal sequence amino acids 526-537 of Human GAD1 (NP_000808.2).

  • 阳性对照
    • Human Brain (Cerebellum); Mouse and Rat Brain lysates

性能

应用

Our Abpromise guarantee covers the use of ab104561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Catalyzes the production of GABA.
  • 组织特异性Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 疾病相关Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 序列相似性Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody 图像

  • Anti-GAD67 antibody (ab104561) at 1 µg/ml + Mouse Brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 67 kDa
  • ab104561 at 4 µg/ml, staining GAD1 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum by Immunohistochemistry using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GAD67 antibody (ab104561)参考文献

ab104561 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 10% donkey serum and 1% BSA in PBS with 0.1% TritonX -100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Sodium citrate buffer pH 6.0
Sample Mouse Tissue sections (Embryonic mouse brain, 5 um thick sections)
Specification Embryonic mouse brain, 5 um thick sections
Permeabilization Yes - 0.3% TritonX-100 in PBS
Fixative Paraformaldehyde
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提交于 May 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"