Anti-GAD65 + GAD67抗体(ab49832)

概述

  • 产品名称Anti-GAD65 + GAD67抗体
    参阅全部 GAD65 + GAD67 一抗
  • 描述
    兔多克隆抗体to GAD65 + GAD67
  • 特异性Reacts specifically with GAD 65 and GAD 67 isoforms from brain.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Human, Pig
  • 免疫原

    Synthetic peptide:

    K-DIDFLIEEIERLGQDL

    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585).

  • 阳性对照
    • Rat brain extract. Rat pancreas tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度Ion Exchange Chromatography
  • 纯化说明Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/1000.

靶标

Anti-GAD65 + GAD67 antibody 图像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.

  • ab49832 staining human pancreas tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C.  An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

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Anti-GAD65 + GAD67 antibody (ab49832)参考文献

This product has been referenced in:
  • González-Gómez M & Meyer G Dynamic expression of calretinin in embryonic and early fetal human cortex. Front Neuroanat 8:41 (2014). Read more (PubMed: 24917793) »
  • Aligny C  et al. Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5:e1311 (2014). ICC/IF ; Mouse . Read more (PubMed: 24991763) »

See all 9 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Rat Tissue (Inhibitory Neurons)
Specification Inhibitory Neurons
Username

Abcam user community

Verified customer

提交于 Nov 18 2015

Thank you for contacting us. We recommend that you use this antibody for IHC-P at a dilution of 1/1000, incuabted overnight. You should first block the tissue with 10% normal serum plus 1% BSA for 1-2 hours to diminish background.

I hope thi...

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable....

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Thank you for contacting us.
I am sorry to hearthat you are encountering some problems with this antibody.

As discussed over the phone, I am sending the link to our antigen retrieval protocols:

http://www.abcam.com/index.html?...

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I am happy you received the antibody already, and hope it works as expected.
If not, please do let us know and we will be happy to assist you further.

Thank you for contacting us.


The anti-GAD65 + GAD67 antibody (ab49832) has to our knowledge not been used with free floating sections as yet and your customer would therefore be eligible for a testing discount. I have generated the code...

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As requested, I have issued a free of charge replacement with the order number XXXXXX from lot XXXXX of ab45429.

To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vi...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (Brain)
Specification Brain
Username

Abcam user community

Verified customer

提交于 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO buffer
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 11 2008

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"