Anti-GAD65抗体[GAD-6] (ab26113)
Key features and details
- Mouse monoclonal [GAD-6] to GAD65
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-GAD65抗体[GAD-6]
参阅全部 GAD65 一抗 -
描述
小鼠单克隆抗体[GAD-6] to GAD65 -
宿主
Mouse -
特异性
The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Mammals -
免疫原
Full length protein (Rat)
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表位
ab26113 recognizes an epitope near the C-terminus of GAD65 -
阳性对照
- mouse brain
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
GAD-6 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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说明 |
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WB
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Catalyzes the production of GABA. -
序列相似性
Belongs to the group II decarboxylase family. -
翻译后修饰
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
细胞定位
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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数据库链接
- Entrez Gene: 14417 Mouse
- SwissProt: P48320 Mouse
- Unigene: 4784 Mouse
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别名
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (70)
ab26113 被引用在 70 文献中.
- Cheng YC et al. Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation. Stem Cell Rev Rep 18:839-852 (2022). PubMed: 35061207
- Zhu X et al. BRN2 as a key gene drives the early primate telencephalon development. Sci Adv 8:eabl7263 (2022). PubMed: 35245119
- Huang R et al. Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depression. J Physiol 600:2973-2999 (2022). PubMed: 35639046
- Koebele SV et al. Evaluating the Cognitive Impacts of Drospirenone, a Spironolactone-Derived Progestin, Independently and in Combination With Ethinyl Estradiol in Ovariectomized Adult Rats. Front Neurosci 16:885321 (2022). PubMed: 35692432
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866