Anti-GAD65抗体[GAD-6] (ab26113)


  • 产品名称
    参阅全部 GAD65 一抗
  • 描述
    小鼠单克隆抗体[GAD-6] to GAD65
  • 宿主
  • 特异性
    The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).
  • 经测试应用
    适用于: IHC-P, WB, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Mammal
  • 免疫原

    Full length protein (Rat)

  • 表位
    ab26113 recognizes an epitope near the C-terminus of GAD65
  • 阳性对照
    • mouse brain



Our Abpromise guarantee covers the use of ab26113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 18467596
WB 1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/100. PubMed: 24246263
ICC/IF Use at an assay dependent concentration. PubMed: 22745816


  • 功能
    Catalyzes the production of GABA.
  • 序列相似性
    Belongs to the group II decarboxylase family.
  • 翻译后修饰
    Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.
  • 细胞定位
    Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 65 kDa glutamic acid decarboxylase antibody
    • DCE 2 antibody
    • DCE2 antibody
    • DCE2_HUMAN antibody
    • GAD 2 antibody
    • GAD 65 antibody
    • GAD-2 antibody
    • GAD-65 antibody
    • Gad2 antibody
    • Glutamate decarboxylase 2 (pancreas) antibody
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate Decarboxylase 65 antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamic Acid Decarboxylase 2 antibody
    • Glutamic Acid Decarboxylase 65 antibody
    • MGC161605 antibody
    • MGC161607 antibody
    see all


  • Mouse monoclonal [GAD-6] to GAD65 staining GAD65-containing nerve terminals around cell bodies of Purkinje cells in the rat cerebellar cortex.
  • Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution + mouse cerebellum at 0.75 µg

    HRP horse anti-mouse IgG at 1/5000 dilution

    Predicted band size: 65 kDa

  • Immunohistochemical analysis of frozen section from Mouse embryo, staining the glomerular layer of the brain. GAD65 is labelled with ab26113 (green) diluted 1:1000. Trb2 is labelled with ab23345 (red).

  • Immunohistochemical analysis of Mouse brain cortex sections, labelling GAD65 with ab26113, diluted 1/100.

    See Abreview


This product has been referenced in:
  • Woodhouse A  et al. Repeat propofol anesthesia does not exacerbate plaque deposition or synapse loss in APP/PS1 Alzheimer's disease mice. BMC Anesthesiol 18:47 (2018). Read more (PubMed: 29699479) »
  • Andersen JV  et al. Impaired Hippocampal Glutamate and Glutamine Metabolism in the db/db Mouse Model of Type 2 Diabetes Mellitus. Neural Plast 2017:2107084 (2017). WB ; Mouse . Read more (PubMed: 28695014) »

See all 31 Publications for this product


Western blot
Human Tissue lysate - whole (human hippocampus)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
used protease inhibitors
human hippocampus
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Jun 25 2015

Thank you for the clarification! Unfortunately the blocking peptide is not available for this particular antibody. Please let me know if I can be of further assistance.

Thank you very much for your reply.

I am sorry to hear that the replacement ab112497 did not work in Western blot. Just to confirm, there were multiple bands in your neuron lysate, and no bands with the rab brain lysate? Were you using the sa...

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Thank you very much for your reply.

I'm sending a free of charge vial of ab112497 on the order **** so that you can compare the results to those with ab26113. This antibody is out of stock but we expect it to arrive around September 27 (next ...

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Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

I actually could not find a reference showing analysis of endogneous GAD65 in culture neurons by Western blot. So, I'm still not quite sure...

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Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. W...

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Thank you for your enquiry regarding ab26113.

It has been reported that GAD65 is transported to nerve terminals and this antibody (ab26113) should stain them as “puncta” around the cell body of their target neurons.

On t...

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Thank you for contacting Abcam.

Based on the protocol the customer sent, there are a few suggestions that may help resolve the issue:

1 - Increase the concentration of primary antibody used to 1/200.

2 - Increase the incub...

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