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Our Abpromise guarantee covers the use of ab85866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|RIA||Use at an assay dependent concentration.|
This image is courtesy of an anonymous Abreview
ab85866 staining GAD65 in murine brain synaptosomes by Flow cytometry.
Cells were homogenized and centrifuged then fixed in paraformaldehyde and permeabilized using PBS Tween. Size gating was used. Primary antibody used at 0.5µg/ml and incubated for 30 minutes at 24°C. The secondary antibody used was an undiluted, Alexa Fluor®488 conjugated goat anti-rabbit IgG.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"