Anti-GABPB2抗体(ab88746)

Abreviews (4)Q&A (3)
Customer reviews and FAQs

概述

  • 产品名称Anti-GABPB2抗体
  • 描述
    小鼠多克隆抗体to GABPB2
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length Human protein (NP_005245.2)

  • 阳性对照

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
IP
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
    • 序列相似性Contains 5 ANK repeats.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • BABPB2 antibody
      • E4TF1 antibody
      • E4TF1-47 antibody
      • E4TF1-53 antibody
      • E4TF1B antibody
      • GA binding protein transcription factor beta subunit 1 transcript variant gamma 2 antibody
      • GA binding protein transcription factor, beta subunit 1 antibody
      • GA binding protein transcription factor, beta subunit 2 antibody
      • GA-binding protein subunit beta-1 antibody
      • GABP subunit beta-1 antibody
      • GABP subunit beta-2 antibody
      • GABP1_HUMAN antibody
      • GABPB antibody
      • GABPB-1 antibody
      • GABPB-2 antibody
      • GABPB1 antibody
      • GABPB2 antibody
      • NRF2B1 antibody
      • NRF2B2 antibody
      • Nuclear respiratory factor 2 antibody
      • Transcription factor E4TF1-47 antibody
      • Transcription factor E4TF1-53 antibody
      see all

    Anti-GABPB2 antibody 图像

    • Western blot - GABPB2 antibody (ab88746)
      Western blot - GABPB2 antibody (ab88746)
      Anti-GABPB2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg

      Predicted band size : 42 kDa
    • Western blot - GABPB2 antibody (ab88746)
      Western blot - GABPB2 antibody (ab88746)
      Anti-GABPB2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg

      Predicted band size : 42 kDa
    • Western blot - GABPB2 antibody (ab88746)
      Western blot - GABPB2 antibody (ab88746)
      Anti-GABPB2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 42 kDa
    • Western blot - GABPB2 antibody (ab88746)
      Western blot - GABPB2 antibody (ab88746)
      All lanes : Anti-GABPB2 antibody (ab88746) at 1 µg/ml

      Lane 1 : lysate from transfected 293T cells
      Lane 2 : lysate from non-transfected 293T cells

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 42 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABPB2 antibody (ab88746)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABPB2 antibody (ab88746)
      ab88746 at 3 µg/ml staining GABPB2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - GABPB2 antibody (ab88746)
      Immunocytochemistry/ Immunofluorescence - GABPB2 antibody (ab88746)
      ab88746 at 10 µg/ml staining GABPB2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.

    Anti-GABPB2 antibody (ab88746)参考文献

    ab88746 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab88746？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab88746 的 abreview 提交 ab88746 的问题

    7 Abreviews or Q&A

    10
    Votes: Highest First

    I tried the protocol suggestions that Abcam offered, but I'm still not seeing any bands in WB using mouse samples.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Western blot abreview for Anti-GABPB2 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human cancer cell lines)
    Loading amount 20 µg
    Specification Human cancer cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Feb 22 2012

    To whom it may concern:



    I am interested in LOXL1 recombinant protein and found one similar product on your website, however I would like to clarify some details before placing the order, can you please provide me some help with i...

    Read More

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab97928 LOXL1 peptide was used to raise the antibody against LOXL1 (ab87748) and it can be used as blocking peptide with studies using http://www.abcam.com/index...

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    Good day! We have customer who is interested in the above protein. please see below for their message:Thank for your help on the phone, here are the information I would like to find out regarding LOXL1 peptide (ab97928). On the product information page...

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    I apologize for being late in getting back to you.
    The peptide ab97928 is in stock. You can place an order any time.
    I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

    Western blot abreview for Anti-GABPB2 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (9L, PC12, RIE-1)
    Loading amount 20 µg
    Specification 9L, PC12, RIE-1
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Jun 30 2010

    Immunoprecipitation abreview for Anti-GABPB2 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK293 cells stably transfected with FLAG-GABPB2)
    Total protein in input 20 µg
    Specification HEK293 cells stably transfected with FLAG-GABPB2
    Immuno-precipitation step Protein G
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    Username

    Abcam user community

    Verified customer

    提交于 Jun 23 2010

    Western blot abreview for Anti-GABPB2 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (MEF F9 GC-1 NIH-3T3)
    Loading amount 20 µg
    Specification MEF F9 GC-1 NIH-3T3
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Jun 16 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"