概述

  • 产品名称
    Anti-GABPB2抗体
  • 描述
    小鼠多克隆抗体to GABPB2
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length Human protein (NP_005245.2)

  • 阳性对照

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
IP
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
    • 序列相似性
      Contains 5 ANK repeats.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BABPB2 antibody
      • E4TF1 antibody
      • E4TF1-47 antibody
      • E4TF1-53 antibody
      • E4TF1B antibody
      • GA binding protein transcription factor beta subunit 1 transcript variant gamma 2 antibody
      • GA binding protein transcription factor, beta subunit 1 antibody
      • GA binding protein transcription factor, beta subunit 2 antibody
      • GA-binding protein subunit beta-1 antibody
      • GABP subunit beta-1 antibody
      • GABP subunit beta-2 antibody
      • GABP1_HUMAN antibody
      • GABPB antibody
      • GABPB-1 antibody
      • GABPB-2 antibody
      • GABPB1 antibody
      • GABPB2 antibody
      • NRF2B1 antibody
      • NRF2B2 antibody
      • Nuclear respiratory factor 2 antibody
      • Transcription factor E4TF1-47 antibody
      • Transcription factor E4TF1-53 antibody
      see all

    图片

    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg

      Predicted band size : 42 kDa
    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg

      Predicted band size : 42 kDa
    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 42 kDa
    • All lanes : Anti-GABPB2 antibody (ab88746) at 1 µg/ml

      Lane 1 : lysate from transfected 293T cells
      Lane 2 : lysate from non-transfected 293T cells

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 42 kDa
    • ab88746 at 3 µg/ml staining GABPB2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
    • ab88746 at 10 µg/ml staining GABPB2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.

    文献

    This product has been referenced in:
    • Gallart-Palau X  et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep 5:14664 (2015). Read more (PubMed: 26419333) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human cancer cell lines)
    Loading amount
    20 µg
    Specification
    Human cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 22 2012

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab97928 LOXL1 peptide was used to raise the antibody against LOXL1 (ab87748) and it can be used as blocking peptide with studies using http://www.abcam.com/index...

    Read More

    I apologize for being late in getting back to you.
    The peptide ab97928 is in stock. You can place an order any time.
    I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (9L, PC12, RIE-1)
    Loading amount
    20 µg
    Specification
    9L, PC12, RIE-1
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 30 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK293 cells stably transfected with FLAG-GABPB2)
    Total protein in input
    20 µg
    Specification
    HEK293 cells stably transfected with FLAG-GABPB2
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Jun 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF F9 GC-1 NIH-3T3)
    Loading amount
    20 µg
    Specification
    MEF F9 GC-1 NIH-3T3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 16 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册