- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-GABPB2抗体
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描述小鼠多克隆抗体to GABPB2
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宿主Mouse
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经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Full length Human protein (NP_005245.2)
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阳性对照
- 购买相配的WB阳性对照Recombinant Human GABPB2 protein
- WB: PC-12 or Jurkat cell lysate; Hela nuclear extracts. IHC: endometrium. ICC/IF: HeLa cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 1 x PBS, pH 7.2 -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab88746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC/IF | ||
| IP |
应用说明
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
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序列相似性Contains 5 ANK repeats.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2553 Human
- Entrez Gene: 14391 Mouse
- Entrez Gene: 499883 Rat
- Omim: 600610 Human
- SwissProt: Q06547 Human
- SwissProt: Q00420 Mouse
- Unigene: 654350 Human
- Unigene: 293266 Mouse
see all -
别名
- BABPB2 antibody
- E4TF1 antibody
- E4TF1-47 antibody
see all
图片
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Western blot - GABPB2 antibody (ab88746)Anti-GABPB2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg
Predicted band size: 42 kDa -
Western blot - GABPB2 antibody (ab88746)Anti-GABPB2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg
Predicted band size: 42 kDa -
Western blot - GABPB2 antibody (ab88746)Anti-GABPB2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 42 kDa -
Western blot - GABPB2 antibody (ab88746)All lanes : Anti-GABPB2 antibody (ab88746) at 1 µg/ml
Lane 1 : lysate from transfected 293T cells
Lane 2 : lysate from non-transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABPB2 antibody (ab88746)ab88746 at 3 µg/ml staining GABPB2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - GABPB2 antibody (ab88746)ab88746 at 10 µg/ml staining GABPB2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.
实验方案
文献
This product has been referenced in:
- Gallart-Palau X et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep 5:14664 (2015). Read more (PubMed: 26419333) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"