Anti-GABPA抗体(ab55052)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克��单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/100.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E4TF 1A antibody
    • E4TF1 60 antibody
    • E4TF1A antibody
    • GA binding protein alpha chain antibody
    • GA binding protein transcription factor alpha subunit 60kDa antibody
    • GA binding protein transcription factor alpha subunit antibody
    • GA-binding protein alpha chain antibody
    • GABP A antibody
    • GABP alpha subunit antibody
    • GABP subunit alpha antibody
    • Gabpa antibody
    • GABPA_HUMAN antibody
    • Human nuclear respiratory factor 2 subunit alpha antibody
    • NFT 2 antibody
    • NFT2 antibody
    • NRF 2 antibody
    • NRF 2A antibody
    • NRF2 antibody
    • NRF2A antibody
    • Nuclear respiratory factor 2 alpha subunit antibody
    • Nuclear respiratory factor 2 subunit alpha antibody
    • RCH04A07 antibody
    • Transcription factor E4TF1 60 antibody
    • Transcription factor E4TF1-60 antibody
    see all

Anti-GABPA antibody 图像

  • Overlay histogram showing HeLa cells stained with ab55052 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55052, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.



  • Predicted band size : 51 kDa
    GABPA antibody (ab55052) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • ab55052 at 10 ug/ml staining GABPA in human Hela cells by Immunocytochemistry/ Immunofluorescence.

  • Immunohistochemical analysis of Human prostate carcinoma tissue, staining GABPA with ab55052.

    Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/100 in PBS) for 1 hour at 21°C. A biotinylated-conjugated donkey anti-mouse polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

Anti-GABPA antibody (ab55052)参考文献

ab55052 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate carcinoma)
Specification Prostate carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
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提交于 Jan 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"