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Rat, Sheep, Rabbit, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GABPA.
This antibody gave a positive signal in NIH3T3 whole cell lysate.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
功能 Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
序列相似性 Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Information by UniProt
E4TF 1A antibody
E4TF1 60 antibody
Anti-GABPA antibody 图像
Western blot - Anti-GABPA antibody (ab109328)
Anti-GABPA antibody (ab109328) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 51 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?) Exposure time : 4 minutes
Anti-GABPA antibody (ab109328)参考文献
has not yet been referenced specifically in any publications.
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