重组Anti-GABARAPL2/GATE-16抗体[EP4808] (ab126607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GABARAPL2/GATE-16抗体[EP4808]
参阅全部 GABARAPL2/GATE-16 一抗 -
描述
兔单克隆抗体[EP4808] to GABARAPL2/GATE-16 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human GABARAPL2/GATE-16 aa 50-150. The exact sequence is proprietary.
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阳性对照
- HeLa, Human fetal brain, Human heart and Human spleen lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 6.50 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP4808 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126607于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
靶标
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功能
Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. -
组织特异性
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. -
序列相似性
Belongs to the MAP1 LC3 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 11345 Human
- Entrez Gene: 93739 Mouse
- Entrez Gene: 100359937 Rat
- Entrez Gene: 64670 Rat
- Omim: 607452 Human
- SwissProt: P60520 Human
- SwissProt: P60521 Mouse
- SwissProt: P60522 Rat
see all -
别名
- ATG8 antibody
- ATG8C antibody
- FLC3A antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GABARAPL2 / GATE-16 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.ab126607 was shown to specifically react with GABARAPL2 / GATE-16 when GABARAPL2 / GATE-16 knockout samples were used. Wild-type and GABARAPL2 / GATE-16 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab126607 被引用在 5 文献中.
- Abdelmoaty MM et al. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. Clin Transl Med 12:e958 (2022). PubMed: 35802825
- Agrotis A et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). PubMed: 30661429
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Chino H et al. Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol Cell 74:909-921.e6 (2019). PubMed: 31006538
- Habisov S et al. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 291:9025-41 (2016). PubMed: 26929408