• 产品名称Anti-GABARAPL1抗体
    参阅全部 GABARAPL1 一抗
  • 描述
    兔多克隆抗体to GABARAPL1
  • 经测试应用适用于: Flow Cyt, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human GABARAPL1 recombinant protein.

  • 阳性对照
    • HeLa cell lysate




Our Abpromise guarantee covers the use of ab94996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50.
IHC-P 1/50 - 1/100.
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 14 kDa.


  • 功能Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • 组织特异性Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • 序列相似性Belongs to the ATG8 family.
  • 翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Anti-GABARAPL1 antibody 图像

  • Anti-GABARAPL1 antibody (ab94996) at 1/100 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 14 kDa
  • All lanes : Anti-GABARAPL1 antibody (ab94996) at 1/500 dilution

    Lane 1 : Human SH-SY5Y whole cell lysate - expressing GFP
    Lane 2 : Human SH-SY5Y whole cell lysate - expressing GFP-GABARAP
    Lane 3 : Human SH-SY5Y whole cell lysate - expressing GFP-GABARAPL1
    Lane 4 : Human SH-SY5Y whole cell lysate - epressing GFP-GABARAPL2

    Lysates/proteins at 30 µg per lane.

    HRP-conjugated Goat anti-rabbit at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by George Allen

    See Abreview

  • Immunohistochemistry analysis of formalin-fixed,paraffin-embedded Human brain tissue labeling GABARAPL1 with ab94996 at 1/50 dilution which was peroxidase-conjugated to the secondary antibody,followed by DAB staining.

  • Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-GABARAPL1 antibody (ab94996)参考文献

ab94996 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (SH-SY5Y cells)
Specification SH-SY5Y cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. George Allen

Verified customer

提交于 Sep 26 2013

Ab94996 has been produced with as immungen the whole molecule. The GABARAPL1 protein has 86% identity with the GABARAP protein. It is therefore difficult to make a prediction. Unfortunately, this antibody has not been tested by the laboratory for its c...

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