Anti-GABARAPL1抗体(ab86503)

概述

  • 产品名称Anti-GABARAPL1抗体
    参阅全部 GABARAPL1 一抗
  • 描述
    兔多克隆抗体to GABARAPL1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human GABARAPL1 aa 36-85 (internal sequence).
    Sequence:

    APKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIP

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • 组织特异性Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • 序列相似性Belongs to the ATG8 family.
  • 翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Anti-GABARAPL1 antibody 图像

  • Anti-GABARAPL1 antibody (ab86503) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 14 kDa

Anti-GABARAPL1 antibody (ab86503)参考文献

ab86503 has not yet been referenced specifically in any publications.

Product Wall

I looked at the alignment of the immunogen sequences of our antibodies against GABARAPL1 with GABARAP. The exact immunogen sequences of ab86497 and ab86503 are unfortunately not available, but the laboratory did an alignment: Blast homology shows that...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"