RabMAb

Anti-GABARAP抗体[EPR4806] (ab109299)

概述

  • 产品名称Anti-GABARAP抗体[EPR4806]
    参阅全部 GABARAP 一抗
  • 描述
    兔单克隆抗体[EPR4806] to GABARAP
  • 经测试应用适用于: ICC, WB, IHC-P, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human GABARAP

  • 阳性对照
    • SH-SY5Y cell lysate, Human fetal brain tissue lysate, Flag-GABARAP, Human heart tissue, Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • 组织特异性Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性Belongs to the ATG8 family.
    • 翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • 细胞定位Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Anti-GABARAP antibody [EPR4806] 图像

    • All lanes : Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution

      Lane 1 : Fetal brain tissue lysate at 10 µg
      Lane 2 : SH-SY5Y cell lysate at 10 µg
      Lane 3 : Flag-GABARAP


      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109299, at 1/100 dilution, staining GABARAP in Human heart tissue by immunohistochemistry.
    • ab109299, at 1/100 dilution, staining GABARAP in Human brain tissue by immunohistochemistry.

    Anti-GABARAP antibody [EPR4806] (ab109299)参考文献

    ab109299 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
    Sample Human Tissue sections (Tendon)
    Specification Tendon
    Permeabilization No
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Oct 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (SH-SY5Y cells)
    Specification SH-SY5Y cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    提交于 Sep 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"