Anti-GABARAP抗体[EPR4805] (ab109364)


  • 产品名称Anti-GABARAP抗体[EPR4805]
    参阅全部 GABARAP 一抗
  • 描述
    兔单克隆抗体[EPR4805] to GABARAP
  • 特异性ab109364 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% immunogen homology AND Abreview 38385 WB data GABARAPL2: pBLAST 93% homology (only 1aa gap of 14aa) AND Abreview 38385 WB data
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human, Silk worm (Bombyx mori)
  • 免疫原

    Synthetic peptide, corresponding to residues in Human GABARAP.

  • 阳性对照
    • Fetal kidney lysate, fetal brain lysate, SH-SY5Y cell lysate, HepG2 cell lysate, human brain tissue, HeLa cells.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/500 - 1/1000. Antigen retriecal is recommended
ICC/IF 1/100 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • 组织特异性Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性Belongs to the ATG8 family.
    • 翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • 细胞定位Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Anti-GABARAP antibody [EPR4805] 图像

    • ab109364 staining GABARAP in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling GABARAP with purified ab109364 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • All lanes : Anti-GABARAP antibody [EPR4805] (ab109364) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 14 kDa
    • ab109364, at 1/500, staining GABARAP in Human brain tissue by immunohistochemistry.
    • ab109364, at 1/100, staining HeLa cells by immunofluorescence.

    Anti-GABARAP antibody [EPR4805] (ab109364)参考文献

    This product has been referenced in:
    • Cappelletti C  et al. Autophagy, inflammation and innate immunity in inflammatory myopathies. PLoS One 9:e111490 (2014). Radioimmunoprecipitation ; Human . Read more (PubMed: 25365350) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (skeletal muscle tissue)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification skeletal muscle tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    提交于 Aug 12 2015

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Silk worm (Bombyx mori) Tissue lysate - whole (Midgut)
    Specification Midgut
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    提交于 Sep 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (13%)
    Sample Human Cell lysate - whole cell (SH-SY5Y cells stably expressing GFP, GFP-GABARAP,)
    Specification SH-SY5Y cells stably expressing GFP, GFP-GABARAP,
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. George Allen

    Verified customer

    提交于 Sep 26 2013

    Application IHC - Wholemount
    Sample Fruit fly (Drosophila melanogaster) Tissue (fat body)
    Specification fat body

    Abcam user community

    Verified customer

    提交于 Sep 19 2013

    Thank you very much for your interest our AbTrial program.

    I performed a comparison of the immunogens for our GABARAP antibodies, and the identity between the immunogen of ab109364 and the silkworm protein looks very promising with ...

    Read More

    Thank you for your reply.

    I wish you good luck with the live reporter experiments.

    I will be happy to issue the discount code for you now, but I think it would be better to do this when you know that you will use it. The discount co...

    Read More

    Thank you very much for your interest in our antibodies.

    According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and mor...

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