Anti-GABA Transporter 1 / GAT 1抗体(ab72448)

概述

  • 产品名称Anti-GABA Transporter 1 / GAT 1抗体
    参阅全部 GABA Transporter 1 / GAT 1 一抗
  • 描述
    兔多克隆抗体to GABA Transporter 1 / GAT 1
  • 经测试应用适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Peptide corresponding to amino acid residues from the C-terminal region of rat GABA Transporter 1/ GAT 1

  • 阳性对照
    • rat hippocampal homogenate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 序列相似性Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • 结构域The PDZ domain-binding motif is involved in the interaction with MPP5.
  • 细胞定位Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Anti-GABA Transporter 1 / GAT 1 antibody 图像

  • Anti-GABA Transporter 1 / GAT 1 antibody (ab72448) at 1/1000 dilution + rat hippocampal homogenate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab72448 staining GABA Transporter 1 / GAT 1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 5% serum) for 16 hours at 4°C. A DyLight®-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

Anti-GABA Transporter 1 / GAT 1 antibody (ab72448)参考文献

ab72448 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Hippocampus)
Permeabilization Yes - 0.1% PBS-Triton X
Specification Hippocampus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (hippocampus))
Permeabilization No
Specification Brain (hippocampus)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mr. Yahyah Aman

Verified customer

提交于 Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 04 2013

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"