Anti-GABA Transporter 1 / GAT 1抗体(ab72448)

概述

  • 产品名称
    Anti-GABA Transporter 1 / GAT 1抗体
    参阅全部 GABA Transporter 1 / GAT 1 一抗
  • 描述
    兔多克隆抗体to GABA Transporter 1 / GAT 1
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Peptide corresponding to amino acid residues from the C-terminal region of rat GABA Transporter 1/ GAT 1

  • 阳性对照
    • rat hippocampal homogenate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 序列相似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • 结构域
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • 细胞定位
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

图片

  • Anti-GABA Transporter 1 / GAT 1 antibody (ab72448) at 1/1000 dilution + rat hippocampal homogenate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab72448 staining GABA Transporter 1 / GAT 1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 5% serum) for 16 hours at 4°C. A DyLight®-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchas...

Read More

Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Permeabilization
Yes - 0.1% PBS-Triton X
Specification
Hippocampus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (hippocampus))
Permeabilization
No
Specification
Brain (hippocampus)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Yahyah Aman

Verified customer

提交于 Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 04 2013

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册