Anti-GABA Transporter 1 / GAT 1抗体(ab426)

概述

  • 产品名称
    Anti-GABA Transporter 1 / GAT 1抗体
    参阅全部 GABA Transporter 1 / GAT 1 一抗
  • 描述
    兔多克隆抗体to GABA Transporter 1 / GAT 1
  • 特异性
    Reacts specifically with a 72 kDa protein.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken
    预测可用于: Human
  • 免疫原

    20 residue C-terminal synthetic peptide (Rat).

性能

应用

Our Abpromise guarantee covers the use of ab426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 序列相似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • 结构域
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • 细胞定位
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

图片

  • All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Barca-Mayo O  et al. Astrocyte deletion of Bmal1 alters daily locomotor activity and cognitive functions via GABA signalling. Nat Commun 8:14336 (2017). IF . Read more (PubMed: 28186121) »
  • Ding XD  et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »

See all 14 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin
Username

Poonam Rani

Verified customer

提交于 Apr 07 2016

Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstelle...

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Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchas...

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Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the ...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

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Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. H...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Mrs. Olga Cravetchi

Verified customer

提交于 Nov 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Kevin Jin

Verified customer

提交于 Nov 19 2010

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded ti...

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Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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