概述

  • 产品名称Anti-GABA Receptor Epsilon抗体
    参阅全部 GABA Receptor Epsilon 一抗
  • 描述
    兔多克隆抗体to GABA Receptor Epsilon
  • 经测试应用适用于: IHC-FoFr, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    A region within synthetic peptide: KNSWKLFQFD FTGVSNKTEI ITTPVGDFMV MTIFFNVSRR FGYVAFQNYV, corresponding to intracellular amino acids 237-286 of Human GABA Receptor Epsilon

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab35971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21084677
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • 组织特异性Expressed in many tissues. Highest levels of expression in adult heart and placenta.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRE sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABA(A) receptor epsilon antibody
    • GABA(A) receptor subunit epsilon antibody
    • Gabre antibody
    • Gamma aminobutyric acid (GABA) A receptor epsilon antibody
    • Gamma aminobutyric acid receptor subunit epsilon antibody
    • Gamma-aminobutyric acid receptor subunit epsilon antibody
    • GBRE_HUMAN antibody
    see all

Anti-GABA Receptor Epsilon antibody 图像

  • ICC/IF image of ab35971 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35971, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab35971 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab35971, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of male, virgin female, G17 pregnant, and 30 d post-partum rat medullary neurons, staining GABA Receptor Epsilon with ab35971 at 1/1000 dilution.
  • Anti-GABA Receptor Epsilon antibody (ab35971) (0.2-1 ug/ml) + Human Placenta

    Predicted band size : 42 kDa

Anti-GABA Receptor Epsilon antibody (ab35971)参考文献

This product has been referenced in:
  • Fatemi SH  et al. Expression of GABAA a2-, ß1- and ?-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder. Transl Psychiatry 3:e303 (2013). WB ; Human . Read more (PubMed: 24022508) »
  • Hengen KB  et al. Increased GABA(A) Receptor e-Subunit Expression on Ventral Respiratory Column Neurons Protects Breathing during Pregnancy. PLoS One 7:e30608 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22303446) »

See all 3 Publications for this product

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