The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
IHC (PFA fixed)
1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
Gamma aminobutyric acid GABA B receptor 2 antibody
Gamma aminobutyric acid type B receptor subunit 2 antibody
Gamma-aminobutyric acid type B receptor subunit 2 antibody
Gb 2 antibody
GPR 51 antibody
GPRC 3B antibody
HG 20 antibody
Metabotropic GABA B receptor subtype 2 antibody
R2 SUBUNIT antibody
Western blot - Anti-GABA B Receptor 2 antibody (ab52248)
All lanes : Anti-GABA B Receptor 2 antibody (ab52248) at 1/300 dilution
Lane 1 : HeLa cell extract; without peptide Lane 2 : HeLa cell extract; with peptide
Predicted band size: 106 kDa Observed band size: 106 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GABA B Receptor 2 antibody (ab52248)This image is courtesy of Karine Thibault, CNRS, Paris, France
Immunohistochemistical detection of GABA B Receptor 2 antibody (ab52248) in PFA fixed Rat Dorsal root ganglion sections. ab52248 was used at 1/300 and incubated for 60 hours @ 4°C in PBS-T 0.3%. Secondary antibody: anti IgG Rabbit Conjugation: Alexa Fluor® 488 (1/1000).
Thibault K et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One9:e91297 (2014).
WB, IHC-Fr, IHC-FrFl
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