概述

  • 产品名称Anti-GABA B Receptor 2抗体
    参阅全部 GABA B Receptor 2 一抗
  • 描述
    兔多克隆抗体to GABA B Receptor 2
  • 特异性ab52248 detects endogenous levels of total GABA B Receptor 2 protein.
  • 经测试应用适用于: IHC-FoFr, IHC (PFA fixed), WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human GABA B receptor.

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab52248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC (PFA fixed) 1/300.
WB 1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ELISA 1/10000.

靶标

  • 功能Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
  • 组织特异性Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
  • 序列相似性Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
  • 结构域Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BcDNA:GH07312 antibody
    • CG6706 antibody
    • CT20836 antibody
    • D Gaba2 antibody
    • FLJ36928 antibody
    • G protein coupled receptor 51 antibody
    • G-protein coupled receptor 51 antibody
    • GAB B R2 antibody
    • GABA B R2 antibody
    • GABA B receptor 2 antibody
    • GABA-B receptor 2 antibody
    • GABA-B-R2 antibody
    • GABA-BR2 antibody
    • GABAB R2 antibody
    • GABABR 2 antibody
    • GABABR2 antibody
    • GABB R2 antibody
    • GABBR 2 antibody
    • Gabbr2 antibody
    • GABR2_HUMAN antibody
    • Gamma aminobutyric acid B receptor 2 antibody
    • Gamma aminobutyric acid GABA B receptor 2 antibody
    • Gamma aminobutyric acid type B receptor subunit 2 antibody
    • Gamma-aminobutyric acid type B receptor subunit 2 antibody
    • Gb 2 antibody
    • Gb2 antibody
    • GH07312 antibody
    • GPR 51 antibody
    • GPR51 antibody
    • GPRC 3B antibody
    • GPRC3B antibody
    • HG 20 antibody
    • HG20 antibody
    • HRIHFB2099 antibody
    • Metabotropic GABA B receptor subtype 2 antibody
    • OTTHUMP00000021776 antibody
    • OTTHUMP00000063797 antibody
    • R2 SUBUNIT antibody
    see all

Anti-GABA B Receptor 2 antibody 图像

  • All lanes : Anti-GABA B Receptor 2 antibody (ab52248) at 1/300 dilution

    Lane 1 : HeLa cell extract; without peptide
    Lane 2 : HeLa cell extract; with peptide


    Predicted band size : 106 kDa
    Observed band size : 106 kDa
  • Immunohistochemistical detection of GABA B Receptor 2 antibody (ab52248) in PFA fixed Rat Dorsal root ganglion sections. ab52248 was used at 1/300 and incubated for 60 hours @ 4°C in PBS-T 0.3%. Secondary antibody: anti IgG Rabbit Conjugation: Alexa Fluor® 488 (1/1000).

Anti-GABA B Receptor 2 antibody (ab52248)参考文献

This product has been referenced in:
  • Thibault K  et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One 9:e91297 (2014). WB, IHC-Fr, IHC-FrFl ; Rat . Read more (PubMed: 24618941) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

提交于 Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"