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Our Abpromise guarantee covers the use of ab55051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
ab55051 staining rat brain (hippocampus) sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde in 0.3% PBS-Tween for 1h. Staining with ab5505 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. GABA-B R1 expressed in the hippocampal neurons.
Immunohistochemistical detection of GABA B Receptor 1 antibody (ab55051) in PFA perfusion-fixed rat spinal cord section. ab55051 was used at 1/500, incubated for 18 hours @ 20°C in PBS-T 0.3%. Secondary antibody: Alexa Fluor® 546 anti-IgG Mouse (1/1000).
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