概述

  • 产品名称Anti-GABA抗体
    参阅全部 GABA 一抗
  • 描述
    兔多克隆抗体to GABA
  • 特异性The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine,D/L-aspartate,1-amino-4-guanidobutane (AGB), D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
  • 经测试应用适用于: IHC-FoFr, IHC-Fr, Immunomicroscopymore details
  • 免疫原

    Chemical/ Small Molecule conjugated to BSA by a Glutaraldehyde linker

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
IHC-Fr
Immunomicroscopy
  • 应用说明This antibody is recommended for thin section post-embedding endogenous content mapping by Light and Electron microscopy immunostaining via High Performance Immunohistochemistry (HPI) (see "Protocols" tab above)

    Recommended user dilution: 1/100
    True Dilution at User Dilution: 1/32000

    High-performance thin section immunostaining using silver-intensified immunogold or fluorescence detection.
    Enhanced detection is possible using streptavidin detection.
    EM applications with 10-40 nm gold GAR IgGs or gold streptavidin.

    OPTIMAL FIXATION: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten).
    MINIMAL GLUTARALDEHYDE: 0.00% using minimum glutaraldehyde Enhanced HPI (EHPI) protocol with 4% formaldehyde (see "Protocols" tab above)

    The hapten is osmium tolerant (deosmication required), therefore the antisera can be used on conventional post-embedding electron microscope immunostaining.

    All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.

    The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.

    Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.
  • 靶标

    • 相关性Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
    • 细胞定位Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
    • 别名
      • 4 aminobutanoic acid antibody
      • GABA antibody
      • Gamma amino butyric acid antibody
      • Gamma aminobutyric acid antibody

    Anti-GABA antibody (ab9446)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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    Thank you for contacting us.

    The immunogen forab9446 uses glutaraldehyde linked to the amino end of GABA.

    ab9446 absolutely requires a free carboxyl and the non-occluded 3 carbon GABA chain for binding.

    It will not bind i...

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    Thank you for your enquiry. For ab9446, the immunogen was GABA-Glut-BSA. I am still looking into the other antibodies for you. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    There is no measurable cross-reactivity with D or L glutamate. Fixed tissue cross-reactivity was tested with known targets at recommended IgG dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine,D/L-aspart...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"