概述

  • 产品名称
  • 描述
    兔多克隆抗体to GABA
  • 特异性
    ab8891 targets conjugated GABA (Gamma-Aminobutyric acid), it does not recognize free GABA.
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-FoFrmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to GABA. Synthetic GABA conjugated to bovine serum albumin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, ddH20
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Fixation of tissue for use with these antibodies should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 17394161

靶标

  • 相关性
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • 细胞定位
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • 别名
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

图片

  • Immunohistochemical analysis of rat brain tissue, staining GABA with ab8891.

    Slices were fixed in a 2% glutaraldehyde, 2% formaldehyde solution before being blocked for 2 h at room temperature in PBS with 0.3% Tween-20, 0.2% BSA and 5% normal goat serum. Samples were incubated with primary antibody (1/200) before detection with a fluorescence-conjugated goat anti-rabbit secondary antibody.
  • Immunofluorescent analysis of Human embryonic stem cells, staining GABA with ab8891.

    Cells were fixed with paraformaldehyde before blocking with 10% goat serum, 1% BSA, 0.1% Triton X-100 for 1 hour. Samples were stained with primary antibody (1/500) overnight at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

文献

This product has been referenced in:
  • Zieger E  et al. Roles of Retinoic Acid Signaling in Shaping the Neuronal Architecture of the Developing Amphioxus Nervous System. Mol Neurobiol N/A:N/A (2017). Read more (PubMed: 28875454) »
  • Castaño J  et al. Fast and efficient neural conversion of human hematopoietic cells. Stem Cell Reports 3:1118-31 (2014). Read more (PubMed: 25458894) »

See all 7 Publications for this product

客户评价及客户问答

The optimal incubation time will depend on how your tissue sections are prepared.

The protocol used for the stain of rat brain tissue shown on the datasheet is from this reference:

Margolis EB et al. Identification of rat ventral tegm...

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Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (early LDT coronal sections)
Specification
early LDT coronal sections
Permeabilization
Yes - TritonX-100
Fixative
Paraformaldehyde
Username

Jason Teem

Verified customer

提交于 Jan 08 2014

Thank you for contacting us.
The antibody has been tested in western blot in conjunction with the antigen as a positive control, in which case the band would is ˜67 kDa as the antigen is made with BSA. Free GABA will not be an option. Whatever is l...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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Thank you for your enquiry. According to the reference available for ab8891, they injected rabbits with a GABA-glutaraldehyde-lysine conjugate. I am still awaiting further information regarding ab17390. I hope this information helps. Please do ...

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The originator of this antibody has informed me that they do not know of any species ab8891 does NOT work in successfully. So, it should work in rat. Please let me know if your customer has any more questions.

This antibody has been tested and characterised for ELISA application. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). I would suggest that perhaps yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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